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For those that want to know more about EHP Labs Oxygreens please see even more information about this one of a kind supplement below, explaining what Oxygreens is, the benefits of Oxygreens and what makes it special: Green Superfood Formula & Ingredients As suggested in the Directions, Acetyl L-Carnitine is best taken with OxyShred to maximize its effectiveness. Optimal stimulant dosage so you're not sitting there so full of energy, sweating and getting jittery While not certified vegan, OxyShred contains no animal-based ingredients. According to vegan certification regulations, none of the EHPlabs products are vegan as they are manufactured in a facility that also processes our whey protein. OxyGreens is the best greens powder on the market, suitable for everyone who wants to improve their health and wellbeing, but struggles with their fruit and vegetable intake. Unlike most greens powders on the market, OxyGreens is also the best tasting greens powder and is the first of its kind to have no grassy or bitter after taste.
Pineapple: Hit the sweet spot with a tropical, fruity aromatic pineapple blended with fresh greens that will remind you of all the goodness of a freshly squeezed morning juice - with no grassy, bitter after-taste. Oxygreens Review Our unique green powder supplement promotes immunity, optimal digestion and nutrient absorption from food, as well as reducing oxidative stress and assisting liver detoxification. It contains a green superfood complex, antioxidants, prebiotic fibre and an array of other wellness ingredients in a convenient 1-scoop serving. Wild Melon: Natural Flavor, Silicon Dioxide, Calcium Silicate, Sucralose, DL-Malic Acid, Turmeric (Color), Sodium Chloride, Citric Acid, Fruit & Vegetable Juice Powder (Color) We created OxyGreens to be the MOST DELICIOUS natural, super greens on the market, enhanced with alkalising forest berries and wholefood prebiotic fibre. Using the current state-of-art in SeSaM, mutant libraries have been prepared for project partners in the OXYGREEN consortium. SeSaM libraries of a Baeyer-Villiger monooxygenases and a cytochrome P450 monooxygenase were created and screened for desired enzyme activities. One of the monooxygenase libraries was used for the benchmarking the advanced SeSaM when compared with a standard epPCR method. To evaluate the monooxygenase libraries, ~500 clones of each library were sent to sequencing to investigate the generated exchanges on the nucleotide level. In addition, ~5000 clones were screened for improved testosterone hydroxylase activity to identify improved monooxygenase variants. As first criteria, the mutational spectra obtained by the two mutagenesis methods were compared. It is known that epPCR libraries generated by Taq polymerase contain a high fraction of AT and TA substitutions. By employing SeSaM that sort of nucleotide exchanges could be reduced. On the other hand, transervsions such as GC that were hardly found for the epPCR were significantly increased in the SeSaM library. By introducing consecutive mutations in a codon the number of possible amino acid exchanges increases. Thus, a larger sequence space can be created by SeSaM. In the epPCR library, no clone (out of 500) was found that was carrying a consecutive mutation. On the other hand, in the SeSaM library 5% of the introduced mutations were consecutive ones. Based on the sequencing results, the SeSaM methodology provides a significantly higher diversity. To finally compare the two methods based on our experience we have set up three criteria: the handling/technical feasibility of the method, the obtainable mutational spectrum and the identification of improved monooxygenase variants. Using these criteria, we conclude that SeSaM is more work intensive in handling than epPCR, but has the advantage of consecutive mutations.
All EHPlabs products are researched, formulated and carefully manufactured to ensure that they deliver results. In one specific case, the KGDOs, the interaction between biocatalysis and cell physiology was investigated extensively and revealed non-obvious interconnections which paved the way for a holistic engineering approach. The resulting whole-cell biocatalyst was able to meet the industrial requirements for a process aimed at the production of a specific target compound. This bioprocess was transferred to the industrial partner REX and is currently being tested on pilot scale. It will soon result in a tangible impact on people’s lives by enabling the efficient production of (novel) chemical commodities in a more sustainable and environmentally friendly way. Such a successful transfer of knowledge and technology between academia and industry sets an example and model for the future. Undeniably, the facilitation of knowledge and technology transfer between academia and industry, and the bridging of the gap between the two is a pivotal objective for the attainment of the scientific and technological maturity that must accompany the socio-economic transition of this decade. OxyShred contains caffeine, and for products containing caffeine you should take a 1 week break after consuming the product for a consecutive 4 week period. With OxyShred, we recommend taking a 1 week break each time you finish a tub. To further increase the screening throughput, emphasis was put at developing a screening method based on fluorescence-activated cell sorting (FACS). Employing FACS, one can routinely sort > 107 clones or cells per hour. Different approaches such as coupling FACS with oxygen sensitive dyes have been evaluated for technical feasibility. Finally, a whole cell ultra-high throughput screening platform for P450 monooxygenases based on flow cytometry was established that is based on the dealkylation of a coumarin derivative. This platform is not only restricted to P450 monooxygenases activity, but can also find applications in metagen
Our newest flavors are Passionfruit and Mango, both of which have been very popular. All the OxyShred flavors are delicious, but it really depends on your taste preference. Our daily greens powder contains a prebiotic fiber complex which includes; Chicory Root (Inulin), Green Kiwi Fruit, Green Banana, and Larch. These superfoods help to nourish the good gut bacteria (microbiome) and promote an overall healthy digestive system which improves nutrient absorption in the gut lining, promotes bowel regularity and reduces stomach bloating. Among the most widely used and relevant chemical reactions are processes that make use of molecular oxygen. We are used to think of oxygen as the molecule that enables respiration. However, one must keep in mind that oxygen is one of the most widely used natural “oxidants” (i.e. molecules that “extract electrons” from another molecule) at the heart of a plethora of most diverse chemical reactions. With the help of oxygen, every organic molecule can be modified. However, it is still a challenge to find a proper catalyst that catalyses such oxidations in a selective manner, without catalysing side reactions while functional at industrial conditions. Enzymes show great promise as selective oxidation catalysts. To exploit oxygen-aided reactions in industrial processes, reliable and robust oxidative enzymes are in high demand. Inulin, Organic Barley Grass, Organic Wheat Grass, Natural Flavours, Citric Acid, Larch (Arabinogalactan), Green Banana, Organic Kale, Organic Spinach, Organic Spirulina, Celery, Kiwi Fruit, Sweetener (Sucralose), Vegetable Gum (Acacia), Silicon Dioxide, Organic Acai, Organic Goji Fruit Extract, Potassium Chloride, Organic Chlorella, Blueberry, Calcium Silicate, Broccoli Seed Extract (13.5% Glucoraphanin), Kelp Laminaria.Starting from crude sample isolation, followed by an HPLC separation based on MS analysis and product isolation via a solid phase extraction unit, the sample was analyzed using a NMR spectrometer for structural analysis. Hence, concentrated fermentation samples were re-dissolved in a mixture of ACN/MeOH and HPLC analysis was conducted. By using an XB-C18 column and an ion trap mass spectrometer it was possible to identify the three main fermentation products. After identification of the desired hydroxylated metabolites via mass spectrometry, structural analysis via NMR spectroscopy was performed to distinguish between different hydroxylation positions. The major hydroxylated product that we were able to isolate and identify, based on HPLC/MS-SPE/NMR analysis and previously published spectral data were 2,17-dihydroxy-(2β,17β)-androst-4-en-3-one. Additional 2-D experiments (COSY and HSQC) and the change of solvent completed the analysis. We created OxyGreens to be the MOST DELICIOUS natural, super greens on the market, enhanced with alkalizing forest berries and wholefood prebiotic fiber. Chicory root is a prebiotic that encourages the growth of healthy gut bacteria. It helps to slow down upper GI tract transit and regulate blood sugar levels, which increases satiety and contributes to weight management ⁵. Kiwi fruit has been demonstrated to be of great benefit for those suffering from high blood pressure, gastritis, and hypertension, amongst other cardiovascular diseases due to its high fibre, potassium and antioxidant content ⁶. Given the gut & skin are closely linked, increasing nutrient intake and supporting gut health will also improve skin health, radiance, and overall well-being. Take the hard work out of reaching your micronutrient requirements, for an incredible taste of the best greens supplement you simply won’t resist! It is the best tasting greens product on the market with no grassy aftertaste. OxyGreens comes in 3 incredibly delicious flavors: Strawberry Margarita, Pineapple & Forest Berries. OxyGreens takes the hard work out of reaching micronutrient requirements.
Chromatographic screening method for dioxygenase mutein screening - An analytical chromatographic method based on the LC-MS technology, that enables semi-quantitative medium throughput analysis of product, substrate, and co-substrate (glucose and a-ketoglutarate) of the KGDO-reaction was developed. Furthermore a product-based, quantitative, colorimetric assay was adapted to 96-well plates and made suitable for high-throughput screening of the KGDO-reaction, allowing a screening for new KDGO-muteins in MTP format. The assay is very specific and highly sensitive, as it can detect down to 1 µM product in the linear range of 1 to 40 µM. With this new screening assay in hand the screening of KGDO-libraries generated in WP2 was performed and new muteins with desired properties were found. After cell lysis, the in-vitro screening procedure based on spectrophotometric determination of hydroxyproline was performed to identify optimized P4H variants from SeSAM library. Muteins with different kinetic characters were identified. By applying the same assay, the obtained muteins were characterized with regard to catalytic efficiency, whereas the industrial partner REX tested the new catalysts during up-scaling. We have carefully selected Chicory Root (Inulin), Green Kiwi Fruit, Green Banana, and Larch as our powerful prebiotic fiber complex for our OxyGreens. These superfoods help to nourish the good gut bacteria (microbiome) and promote an overall healthy digestive system. This will improve nutrient absorption in the gut lining, as well as promoting bowel regularity and reducing stomach bloating. You will feel lighter and more energetic. Cell-based screening methods -To generate designer enzymes by protein engineering comprises three subsequent steps: efficient generation of diversity on the protein level, efficient expression of the target enzyme in the expression host and an efficient screening system to identify novel enzymes with desired features. The availability of an appropriate screening system is essential in protein engineering experiments (“You get what you screen for!”) and is thus determining the success of such approaches. Many important redox enzymes need NAD based cofactors, which are efficiently regenerated by the cellular metabolism. In addition whole cells can also act as a cover protecting the enzymes from shear forces or organic solvents and, thus, increase their stability. On the other hand such the protecting cell sometimes limits substrate access to the intracellular enzymes. Another advantage of cell based screenings is that enzyme isolation and purification steps can be circumvented which speeds up the screening process. Therefore to speed up breeding cycles, to reduce costs and to simulate later processes as far as possible, work focused on the development of whole cell screening systems. The OXYGREEN-project aimed at obtaining simple, novel, and faster enzyme redesign approaches by advancing methods for diversity generation and knowledge-driven approaches in sequence-alignment and structure-guided mutein (=mutant enzyme) design. Novel screening technologies allowing accurate measurements and mutein evaluation in high-throughput will lead to a breakthrough in biocatalyst platform development over a short time scale.
The nucleoside ribavirin (dRTP) is a well-known antiviral drug for hepatitis C. The base functions as purine analog and pairs thus with T and C. Novel nucleotide analogs have been prepared and were investigated for the applicability in SeSaM. It was shown that dRTP can be added to the 3´-end of a fluorescence labeled oligonucleotide by the enzyme terminal transferase and represents a “better substrate” for the terminal transferase than dPTP. Furthermore, the engineered DNA polymerase, compared to Taq-polymerase and vent (exo)-polymerase, is able to elongate a mismatch. With the implementation of these improvements, the advanced SeSaM protocol (named SeSaM-P/R) allows for the first time to introduce at all possible positions transversion mutations, which are homogeneously distributed over the targeted gene. With this, the method comes close to the perfect method as it allows altering each nucleotide of a gene. Advancements compared to SeSaM-P lie in the more diverse fragmentations patterns, omitting the use of template-DNA, doubling the mutation frequency, and chemical diversity due to parallel employment of the P- and R-base. The SeSaM-P/R method nearly doubles the number of by epPCR unobtainable amino acid substitutions when compared to previous SeSaM methods. Transversions cause amino acid substitutions that are naturally not or barely occurring leading to chemically diverse amino acids substitution patterns. The latter mutational bias might become beneficial for protein reengineering challenges such as improving solubility of proteins in water, increasing thermal resistance or stability in organic solvents. Summarizing, the SeSaM-technology was successfully optimized towards minimization of unmutated sequences, more transversions and more consecutive mutations in resulting SeSaM-libraries.
Antioxidants also help combat free radicals to promote glowing, healthy skin and boost immunity. Prebiotic fibre contributes to healthy digestion and staying regular.
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Boost Immunity & Vitality: Additional greens including; Celery, Spirulina, Chlorella and Kelp have been added into this green superfood powder. They are rich in vitamins, minerals, iron and chlorophyll to help maintain a healthy digestive function and support overall wellbeing. Studies suggest Spirulina may also help to maintain a healthy immune system. OxyShred uses the sweetener Sucralose, as it's been FDA-approved for 20 years, and shown to be safe and stable. Sucralose has been extensively studied and more than 110 safety studies were reviewed by FDA in approving the use of sucralose as a general purpose sweetener for food. Finally, also the detection of hydrogen peroxide by using the Peroxy Green 1 dye has been implemented in the assay. This allows for the identification of so-called uncoupling mutants which consume NADPH without catalyzing the Baeyer-Villiger reaction and produce hydrogen peroxide instead of valuable target products. By adding this assay method, it is possible to discern false positives. In the logic of the project, the development of tools for creating mutant enzyme collections is paralleled with the development of tools to effectively evaluate the properties of the enzymes and their mutated variants. Most important, a key aim of this part of the project was to develop methods that can be used in a high-through put manner. Only with such methods, large libraries of mutant enzymes can be assayed for desired enzyme activities. The project has yielded several improved or new methods for screening for desired enzyme activities. Generic medium-throughput technologies had to be developed to provide novel screening platforms for oxygenases for instance electrochemical microtiter plate screening for P450 monooxygenases, enzyme-coupled screening for Baeyer-Villiger monooxygenases and a product-based screening for α-ketoglutarate dependent dioxygenases. All these medium-throughput screening technologies had to be developed to enable detection of improved muteins with superior catalytic properties with a throughput of >1000 mutants/day. One key target during development of screening platforms is to liberate cytochrome P450 enzymes from the need of costly coenzymes (NADPH) and to evolve self-sufficient BVMOs. New screening assays and technologies will be used for screening enzyme mutant libraries, including libraries that had been prepared within the project, e.g. SeSaM-based mutein libraries.
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